Q1: What is protein oxidative modification?
A1: Reactive oxygen and nitrogen species (ROS and RNS, respectively) are capable of producing a wide range of post-translational protein modifications. Since protein modifications by ROS/RNS formally alter thiol redox state or otherwise involve redox chemistry, they are referred to as redox-based or redox-related modification.

Q2: What is the biological significance of protein redox modification?
A2: Protein redox modification is of important roles for various types of cellular processes. Moreover, many diseases, such as Parkinson's disease, Alzheimer's disease, are associated with protein redox modification.

Q3: What types of protein redox modification are included in RedoxDB?
A3: RedoxDB includes cysteines of various types of redox modifications, such as reversible disulfide, sulfenic acid, S-nitrosylation, S-glutathionylation, etc.

Q4: Does RedoxDB contain data from proteomics studies?
A4: Yes. RedoxDB only contains data from both case-by-case studies and proteomic studies. You can choose the source of data when searching RedoxDB.

Q5: Links to PDB are provides for many redox proteins in RedoxDB. Why the position of their redox-sensitive cysteines in PDB and in RedoxDB are not always consist with each other?
A5: The protein sequences in RedoxDB are according to UniProt and usually of full-length sequence. However, many proteins in PDB are of partial sequence, thus the position of cysteines are not always identical.

Q6: Why some redox proteins do not appear in RedoxDB?
A6: Currently, RedoxDB only includes redox proteins for which the exact cysteine sites undergone oxidative modification have been experimentally determined. Moreover, since many genes (particularly those from eukaryotes) are of many synonyms, it's possible that keyword searching will return no result although the proteins are included in RedoxDB. For such case, you can try to search by BLAST.

Q7: How could I submit new data to RedoxDB?
A7: If you want deposit your data in RedoxDB, please email mingansun@gmail.com or djguo@cuhk.edu.hk. In the email, please provide gene name, citation, protein sequence, and the position and type of modification (if known) for the redox cysteines.

cuhk_logo Contacts: djguo@cuhk.edu.hk mingansun@gmail.com